Immune Response and Western Blotting
Literature selected by Kathleen M. Dickson
and presented Oct. 9, 1999 for comment at the Lyme-L
Infect Immun 1994 Jun 62:6 2625-7
Tick transmission of Borrelia burgdorferi to inbred strains of mice induces
an antibody response to P39 but not to outer surface protein A
[published erratum appears in Infect Immun 1995 Mar;63(3):1146]
Golde WT, Kappel KJ, Dequesne G, Feron C, Plainchamp D, Capiau C, Lobet
Natural tick transmission of infection by Borrelia burgdorferi induces
a very different serum antibody response than needle inoculation of spirochetes.
We present data, obtained by using the mouse model, that show that the
OspA response was barely detectable, whereas all animals developed significant
anti-P39 titers after exposure to B. burgdorferi-infected ticks.
Infect Immun 1995 Dec 63:12 4795-801
Variation in antigenicity and infectivity of derivatives of Borrelia burgdorferi,
strain B31, maintained in the natural, zoonotic cycle compared with maintenance
Golde WT, Dolan MC
The original isolate of Borrelia burgdorferi, strain B31, can be maintained
in vitro indefinitely. A number of studies have demonstrated that there
are recognizable changes in the genetic composition of the spirochete after
more than 60 passages. We have maintained B31 in the natural zoonotic cycle
of transmission of infection between laboratory mice and laboratory-reared
To determine whether similar changes occur in the natural transmission
cycle, we reisolated strain B31 from mouse skin at the 5. zoonotic
cycle. This reisolated derivative
Analysis of antigen expression with monoclonal antibodies generated
against B31, however, showed differential expression of a subset of antigens
between B31 and the isolated derivative.
had the same infectivity as the parent B31 strain,
had lost the 8-kb supercoiled plasmid present in B31, and
induced a gross serum antibody response indistinguishable from the B31
J Exp Med 1993 Jan 1 177:1 9-17
The major histocompatibility complex-restricted response of recombinant
inbred strains of mice to natural tick transmission of Borrelia burgdorferi.
Golde WT, Burkot TR, Sviat S, Keen MG, Mayer LW, Johnson BJ, Piesman J
The causative agent of Lyme disease, Borrelia burgdorferi, is transmitted
by ticks of the Ixodes ricinus complex.
In this study, we report the antibody response of recombinant inbred
strains of mice of the H-2b, H-2d, and H-2k
haplotypes, infected with B. burgdorferi as a result of exposure to infected
The patterns of antibody response assayed by Western blot analysis indicate
significant major histocompatibility complex (MHC) restriction to bacterial
antigens within the first 2 mo of infection in mice (i.e. during this period
only certain haplotypes have contributed to the immune response, J.
Other bacterial antigens induce a significant response across the MHC haplotypes
when assayed on the same bacterial strain used to transmit the infection
(homologous strains, J.
but do not crossreact with the same proteins derived from heterologous
strains of B. burgdorferi.
No response to OSPA was detected at any time during the 60-d period we
analyzed this infection.
A third group of bacterial antigens appear to generate a MHC-nonrestricted
response, and this lack of restriction is maintained when assaying the
crossreactivity of the response with other strains of B. burgdorferi. These
proteins may provide more accurate diagnostic probes than those currently
Finally, there appears to be a significant difference in the expression
of most bacterial antigens when the spirochete is cultured for many
passages since the same strain of bacterium isolated from low-passage and
high-passage preparations exhibit different banding patterns in Western
blots when assayed with the same sera. The same is true for patient infections.
Version: March 11, 2000.
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